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KMID : 0603820090150040335
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.335 ~ p.342
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Physiological Activities of Roots Extracts from Calystegia japonica
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Lee Yang-Suk
Joo Eun-Young
Kim Nam-Woo
Choi Bok-Dong
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Abstract
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This study was investigated to evaluate the contents of polyphenols and flavonoids, and physiological activities of various extracts from Calystegia japonica roots for making good use of their functional materials. The roots of C. japonica were extracted with water (WE), ethanol (EE) and hot water (HWE) by different methods. Among these extracts, the highest extracting yield was 30.30% of HWE, while the highest contents of total polyphenols and flavonoids were 40.85% and 6.40% of WE, respectively. The nitrite scavenging abilities were ranged from 31.31% (HWE) to 37.46% (EE) at pH 1.2 and 1.0 ㎎/㎖ concentration. In the measurements of electron donating abilities, EE showed the highest effect as 91.83% at 0.3 ㎎/㎖ assay concentration, and the electron donating ability was decreased as the extract concentration was increased. In the results of superoxide dismutase (SOD)-like activity, HWE showed the highest effect as 7.15% at 1.0 ㎎/㎖. The tyrosinase inhibition activities of WE and EE were 15.28% and 14.97%, respectively. The xanthine oxidase inhibitory effects were ranged from 97.50 to 99.28% at 1.0 ㎎/㎖. These results indicate that C. japonica extract has a good antioxidant effects and could be useful for developing functional products.
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KEYWORD
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Calystegia japonica, Nitrite scavenging, Electron donating, SOD-like ability, Tyrosinase, Xanthine oxidase
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